This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Enterococcus spp. by conventional cultural and molecular identification using PCR and partial sequencing of 16S rDNA techniques. Out of 104 samples, 73 (70.2%) isolates were found to be enterococci based on their cultural characteristics on ESD medium. However, out of 36 samples subjected to molecular identification, only six isolates were confirmed to be Enterococcus spp. using PCR and partial sequencing of 16S rDNA technique. All enterococci strains tested for their antibiotic sensitivity profiles showed high percentage of multi-resistance phenotype. These results can be used for further studies on enterococci as an emerging food borne pathogen and its role in human infection in Libya and would suggest that meat, meat products and seafood might play a role in the spreading of enterococci through the food chain with antimicrobial resistance characteristics.
Ibrahim Eldaghayes(6-2017)

A total of 154 of Escherichia coli (E. coli) isolates were isolated from faecal specimens of healthy cattle (n=100). Serotyping of 154 E. coli isolates indicated that 112 (72.72%) isolates were typeable, 28 (18.18%) untypeable and 14 (9.09%) were rough. The most predominant serotype observed was O22 followed by O56, O60, O120 and O1. Antibiogram pattern using a total of 25 different antibiotics indicated that high resistant was present against clindamycin, metronidazole and penicillin followed by cephalothin, neomycin, kanamycin, cephalexin, streptomycin, furazolidone and tetracycline antibiotics. The amplification of tet(A) and tet(B) resistant determinants by PCR resulted in generation of 372bp and 228, respectively. The tet(A) gene was predominant gene compared to tet(B) gene. This study using pheno-genotypic characterization indicated the presence of antimicrobial resistant E. coliisolates amongst healthy cattle, emphasizing that effective strategy should be applied to persist the efficiency along with ideal usage of novel antibiotics though minimizing the risk of antibiotic resistant bacteria. arabic 11 English 82
Abdusalam Sharef Abdusalam Mahmoud(3-2020)

In order to study microscopic and macroscopic lesions in chickens infected with low and high doses of Salmonella enteritidis PT 4 (SE), 150 one-day-old White Plymouth Rock chicks were divided into three groups each containing 50 birds. The chickens of the first and second groups were inoculated with 2 x 102 and 2 x 108 cfu of Salmonella, respectively. The chicks of the third group were kept as uninoculated control birds. Five birds from each group were euthanized by cervical dislocation at 6, 12, 18 hours post inoculation (hpi) and then after 1, 3, 7, 10, 14, 21, and 27 days post inoculation (dpi) and were examined for presence of gross and histological lesions. Catarrhal typhlitis and unabsorbed yolk sac were the most prevalent gross lesions in both groups. Histologically, granulomatous nodules in the caecum were found occasionally in some cases in both inoculated groups. The present study demonstrated that the chickens infected with a low dose of SE showed fewer lesions, which were milder in comparison with the birds inoculated with a high dose of SE. It suggests that chickens were able to capture the pathogen in the low dose group
Abdulatif Asheg(1-2001)