The GC-box is an important transcriptional regulatory element present in the promoters of many mammalian genes, and is found in most, if not all, oxidative phosphorylation (OXPHOS) promoters. In the present study we examine the effects of three Sp1 family members (Sp2, Sp3, and Sp4) on the adenine nucleotide translocase 2, cytochrome c1, F1-ATPase β-subunit, and the mitochondria transcription factor (mtTFA) promoters in Drosophila SL2 cell line. Sp3, like Sp1, strongly activates transcription all four promoters. SP4 stimulates, moderately, but Sp2 had no effect. In addition, Sp3 can, like Sp1, inhibit transcription from the proximal promoter of the ANT2 gene through binding to the Cbox GC element. By contrast, Sp4 and Sp2 do not repress promoter activity. Furthermore, since Sp4 and Sp2 bind to the Cbox repression element on the ANT2 promoter, but do not repress transcription, inhibition of transcription … arabic 21 English 97
Ahmed Zaid(1-2012)

Fungi are a huge source of unexplored bioactive compounds. Owing to their biological activities, several fungi have shown commercial application in the health industry. Tuber aestivum Vittad. is one such edible fungi with an immense scope for practical biological applications. In the present study, the anti-angiogenic activity of petroleum ether and ethanol extracts of T. aestivum was investigated using the chick chorioallantoic membrane assay and compared to the positive controls silibinin and lenalidomide. Both the extracts showed a dose-dependent anti-angiogenic response. The extracts were also assessed for their anti-inflammatory potential by lipoxygenase-inhibition assay. The IC50 values for LOX inhibition assay, computed by the Boltzmann plot, were 368.5, 147.3 and 40.2 μg/mL, for the petroleum ether extract, ethanol extract, and the positive control ascorbic acid, respectively. The ethanol extract of T. aestivum showed superior anti-angiogenic and anti-inflammatory activity than the petroleum ether extract. Compositional investigation of the extracts by GC–MS revealed the presence of various bioactive compounds. The compounds were correlated to their anti-angiogenic and antiinflammatory activity based on a meticulous literature search. arabic 25 English 145
Sandesh J. Marathe, Abdulla Bashein, (1-2020)

Background: Atopic dermatitis (AD) is a common, chronic, relapsing, itchy, skin condition occurring in patients with a personal or family history of atopy, and there is clinical association among different allergic disease in a way that treating one of them will improve the other. Many studies worldwide showed presence of AD in asthmatic children with different prevalence among countries and showed clinical improvement in asthma control on treatment of atopic dermatitis. Therefore, this study was conducted to assess the prevalence of atopic dermatitis among Libyan asthmatic children. Methods: This is an observational cohort study on asthmatic Libyan children who were treated and followed up at Tripoli children hospital in Tripoli, Libya. It carried out on 300 children suffering from asthma admitted from pediatric outpatient department as well as from emergency department and asthma clinic over a period of 24 months; from December 2017 to December 2019. The parents were asked to complete a questionnaire to collect the needed information after their consent being taken. To assure the accuracy and consistency of the methodology (sampling procedure, measurements, and a collection of the data), a standardized protocol was prepared. Data were entered in SPSS statistical package and consequently were analyzed and presented as descriptive statistics. Results: The prevalence of atopic dermatitis among asthmatic Libyan children was 16.7% in our study. The results showed significant relationship between address and prevalence of atopic dermatitis. Conclusion. Further studies are required to address the ethnicity, environmental factors, skin type and others attributed to this problem and we recommend all pediatricians to look for AD in asthmatic children and treat it accordingly arabic 7 English 56
Hisham Alrabty, Munera Addala(5-2020)